What describes the concept of alternatives in protocol review?

• A. The cost of the protocol.
• B. Alternatives to animal use, such as in vitro methods or computer modeling.
• C. The color of the cages.
• D. The location of the facility.

Answer: (B)

In protocol review, the main concept being tested is whether there are viable alternatives to using animals, such as the mouse, by employing non-animal methods like in vitro assays or computer modeling. Reviewers assess whether these non-animal approaches can address the same scientific questions or whether animal use is truly necessary. This reflects the commitment to the 3Rs—replacing animals with non-animal methods when possible, reducing the number of animals used, and refining procedures to minimize suffering. The other factors mentioned—cost, cage color, or facility location—don’t relate to the availability or appropriateness of alternatives to animal use, so they aren’t part of this concept.