When restraining a mouse for a brief procedure, which approach is appropriate?

• A. Grasp the neck and immobilize the head
• B. Allow the mouse to move freely
• C. Support the body and restrain only as needed
• D. Hold the hind legs rigidly

Answer: (C)

Restraint during mouse handling should minimize stress and risk while still allowing the procedure to be performed. The best approach is to support the body and restrain only as needed. This means cradling the mouse to stabilize the torso and prevent sudden movements, using gentle pressure that keeps breathing comfortable and avoids constricting the chest. Restraining only for the minimum time and amount necessary helps reduce distress for the animal and lowers the chance of injury to both the mouse and the handler. Grasping the neck and immobilizing the head tends to cause unnecessary stress and can injure sensitive neck structures. Letting the mouse move freely during a procedure defeats the purpose of restraint and can lead to escapes or accidental harm. Holding the hind legs rigidly imposes an uncomfortable, rigid position and increases the risk of musculoskeletal injury. In short, support the body and restrain only as needed to complete the task safely and humanely.