Which statement about monoclonal antibody production methods is true?

• A. In Vivo Techniques are used.
• B. In Vitro Techniques are the recommended method.
• C. In Vitro Techniques are incorrect.
• D. Monoclonal antibodies cannot be produced.

Answer: (B)

Producing monoclonal antibodies relies on creating a single antibody-producing cell lineage and expanding it under controlled conditions. This is done in vitro, typically by fusing an antibody-secreting B cell with a myeloma cell to create a hybridoma, or by using recombinant DNA techniques in cell culture. The result is a clone-derived population that makes identical antibodies with a single specificity. In vivo approaches involve immune responses in a living animal and typically yield polyclonal antibodies from many B cell clones, not monoclonal. Therefore, the in vitro method is the standard and correct approach for true monoclonal antibody production.