Which statement about oral gavage technique in small mice is accurate?

• A. A straight needle is preferred to prevent trauma to the esophagus
• B. A curved needle is preferred for better control
• C. Gavage is contraindicated in all mice under 20 g
• D. Any needle size is acceptable

Answer: (A)

When performing oral gavage in very small mice, the goal is to deliver the slurry into the stomach with minimal trauma to the delicate esophageal and airway tissues. A straight gavage needle is preferred because it follows the natural axis of the esophagus, providing a smooth, direct path to the stomach. This reduces the chance of the tip catching mucosa, bending into the wrong tract, or causing uncontrolled tearing or perforation. In contrast, a curved needle can be more likely to snag tissue or misdirect the tip, increasing trauma risk—particularly in tiny animals where tissue is fragile.

In addition, use a properly sized needle with a rounded, not sharp, tip and insert only to the appropriate depth to avoid injury. It’s not correct that any needle size is acceptable; using an inappropriate size can cause trauma or fail to deliver the dose properly. It’s also not correct that gavage is contraindicated in all mice under a certain weight; with proper technique and restraint, small mice can be gavaged safely.